Tissue culture technology is an emerging technology that has only developed in recent years. This technology can increase plant growth efficiency and provide a better research platform for biological research. After decades of development, plant tissue culture technology has made great progress. Gradually, people's research has begun to turn to culture conditions, such as culture temperature, light intensity, air inside the container, plant growth during tissue culture and cultivation. Speed, quality of growth effects. Here we analyze the impact of these specific factors on plant tissue culture. The control of illuminance and other parameters in the tissue culture process requires the use of hand-held meteorological stations and light incubators. These instruments are able to sense changes in light, temperature, etc. in the environment in a timely manner.
Illumination in tissue culture is also one of the important conditions, mainly in terms of light intensity, light quality, and illumination time: 1. Light intensity: Light intensity has an important influence on the proliferation of cultured cells and the differentiation of organs, from the current research situation. Look, the light intensity has obvious influence on the initial division of the external plant body and cell. In general, the light intensity is strong, the growth of the seedling is thick, and the light intensity is weak and the seedling is easy to grow. The control of the light intensity can be achieved through the light incubator, because the light box can manually set the light intensity and light time we need. 2. Light quality: Light quality has a significant effect on the induction of callus, the proliferation of cultured tissues and the differentiation of organs. For example, lily buds were cultured under red light. After 8 weeks, callus was differentiated. However, the callus appeared only after a few weeks of culture under blue light, but buds emerged after 15 days of inoculation with gladiolus. Under blue light, buds emerged first. The seedlings grew vigorously and the seedlings became fine under white light. According to Ni Dexiang and others in the research of carnation, the growth amount under the white light conditions is the highest, followed by red, yellow, green and blue light which have inhibitory effects on the growth. The monochromatic light has the inhibitory effect on the chlorophyll synthesis, and the chlorophyll synthesis needs to be in the complex light conditions. Completed. 3, the photoperiod: plantlet culture to use a certain period of light and dark for tissue culture, the most commonly used cycle is 16h light, 8h dark. Studies have shown that the organs of species sensitive to short days are easy to differentiate under short days, but produce callus under long daytime and sometimes require dark culture, especially for callus of some plants under dark culture than under light It's better. Such as safflower callus.
In addition to the dark conditions required for some materials to induce callus, general culture requires a certain amount of light.
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